Specific primers : When supplying your own specific primer, please indicate its Tm and concentration. Primer Sequence Catalog no. bgh reverse primer: gctgg caact agaag gcaca g: pcold-f1 primer *7: gtaag gcaag tccct tcaag ag: pcold-r primer *7: cgcat tctca ttgca cccaa: pcoldtf-f1 primer *7: ccact ttcaa cgagc tgatg: rv-p: ggaaa cagct atgac catga ttac: m13-20: cgacg ttgta aaacg acggc cagt: m13-47b *8: ggcga aaggg ggatg tgctg caag: 10f *9: gtttg atcct ggctc a: Primers on the Standard Primer List (below) are provided free of charge. Primer Sequence pMoles Supplied T7 Promoter 5´-TAATACGACTCACTATAGGG-3´ 328 BGH Reverse 5´-TAGAAGGCACAGTCGAGG-3´ 358 Genotype of TOP10 Cells It binds to a wide variety of DNA templates. Store resuspended primers at –20°C. Primer sequences can be found here: M13 Forward GTA AAA CGA CGG CCA GTG M13 Reverse GGA AAC AGC TAT GAC CAT G T7 Promo If necessary, the shorter version of SP6 is available 5'-CACATACGATTTAGG-3. BGH (bovine growth hormone) terminator, reverse primer. This plasmid is available through Addgene. Primers should be provided at a concentration of 10µM (picomoles/µl). Universal primers are complementary to nucleotide sequences used for the amplification of a very similar gene that related to a specific Genus. EGFP-C Primer Sequence M13 Forward (-20) 5'{GTA AAA CGA CGG CCA G}3' M13 Reverse (-20) 5'{CAG GAA ACA GCT ATG AC}3' SP6 5'{ATT TAG GTG ACA CTA TAG}3' T3 5'{ATT AAC CCT CAC TAA AGG GA}3' T7 Promoter 5'{TAA TAC GAC TCA CTA TAG GG}3' T7 Terminator 5'{GCT AGT TAT TGC TCA GCG G}3' pcDNA3.1/BGH Reverse 5'{TAG AAG GCA CAG TCG AGG}3' 5'-pGEX 5'{GGG CTG GCA … Resuspension: Resuspend sequencing primers in sterile water to a final concentration of 0.1 µg/µl. These free universal primers are being updated to reflect the needs of our customers. Plasmid Preparation 2 µg/μL in TE buffer, pH 8.0 . 5′ end of ampicillin resistance gene, reverse primer: AUG1 Forward: CAATTTACATCTTTATTTATTAACG For Pichia vectors with AUG1 promoter, forward primer: AUG1 Reverse: GAAGAGAAAAACATTAGTTGGC For Pichia vectors with AUG1 promoter, reverse primer: BGH Reverse: TAGAAGGCACAGTCGAGG Bovine growth hormone terminator, reverse primer: … It must be provided in a separate tube at 10 uM. CMV-F. CGCAAATGGGCGGTAGGCGTG. The pcDNA ™ 3.4-TOPO ® TA Vector Kit contains the following primers to sequence your insert. Customer Provided Primers. CMV Forward 5´-CGCAAATGGGCGGTAGGCGTG-3´ N622-02 BGH Reverse 5´-TAGAAGGCACAGTCGAGG-3´ N575-02 General Molecular Primer Sequence Amount T7 5´-TAATACGACTCACTATAGGG-3´ 328 pmoles GFP Reverse 5´-GGGTAAGCTTTCCGTATGTAGC-3´ 296 … Primer Sequence The subsequent recombinant PCR using CMV forward primer, located upstream of the cDNA sequence, and BGH reverse primer, located downstream of the cDNA sequence has been performed to fuse the overlapping mutant fragments. Bioz Stars score: 89/100, based on 73 PubMed citations. (2) Formerly named New-SP6. Primers . - MALDI-TOF QC - Confirms purification by HPLC - 70 types of primers for sequencing, 17 types of primers for microbe identification and 5 types of random primers primers sequences; 3’ ad: 5’ (aga tgg tgc acg atg cac ag) 3’ 3 aox1: 5′ (gca aat ggc att ctg aca tcc) 3’ 5’ ad: 5’ (ttc gat gat gaa gat acc cc) 3’ 5 aox1: 5′ (gac tgg ttc caa ttg aca agc) 3’ bgh reverse: 5′ (tag aag gca cag tcg agg) 3′ bk reverse: 5′ (aca gga aac agc tat … For your convenience, we offer a custom primer synthesis service. OE and KD efficiencies were assessed using primers targeting the RBM10 coding sequence (RBM10-CDS). As the largest gene synthesis provider in the USA with proven capability and reliability, GenScript now expands DNA sequencing services in North America to offer … BGH Reverse primers to confirm that your gene is in the correct orientation for expression and contains an ATG and a stop codon. DuetDOWN1: GATTATGCGGCCGTGTACAA: For pETDuet, pACYCDuet vectors (7) These primers work in the Duet vectors for co-expression of proteins. ZERO BIAS - scores, … suggest using the T7 Promoter and BGH Reverse primer sequences. Bioz Stars score: 95/100, based on 37 PubMed citations. For more information, refer to www.lifetechnologies.com or contact Technical Support (see page 12). Sequences. Refer 3–5 for the sequence and location of the priming sites. Primer Map Restriction endonuclease cut sites, and the protein translations of the DNA sequence can also be shown. Primer Sequence to the diagrams on pages We recommend that you sequence your construct with the T7 Promoter and BGH Reverse primers (see page 12 for ordering information) to confirm that your gene is fused in frame with the V5 epitope and the C-terminal polyhistidine tag. Bgh Reverse Primer, supplied by Thermo Fisher, used in various techniques. 2018 May 29. pii: nbt.4172. 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